Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the invasive ability of HCT-116 cells. (A) Microscopic observation and photography after crystalline violet staining (100× magnification); (B) the OD values of each group at 570 nm wavelength after dissolution of crystalline violet by sodium acetate; (C-G) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction; OD, optical density.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Staining, Dissolution, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Journal of Gastrointestinal Oncology

Article Title: Expression and clinical significance of miR-17-5p in tumor tissues of patients with colorectal cancer

doi: 10.21037/jgo-22-1185

Figure Lengend Snippet: The effects of miR-17-5p on the apoptosis of HCT-116 cells. (A,B) Flow cytometric detection of the effects of different transfection groups on apoptosis of HCT-116 cells; (C-I) the relative mRNA and protein expression of miR-17-5p in HCT-116 cells was detected with RT-qPCR and Western blot, respectively. *, P<0.05; **, P<0.01; ***, P<0.001. NC, negative control; in-NC, inhibitor negative control; RT-qPCR, real-time fluorescence quantitative polymerase chain reaction.

Article Snippet: The miR-17-5p overexpression mimics, inhibitor, and corresponding controls, hairpin miRNAs quantification kits, and U6 calibration real-time fluorescence quantitative PCR (RT-qPCR) kits were purchased from Genepharma Co. (Shanghai, China).

Techniques: Transfection, Expressing, Quantitative RT-PCR, Western Blot, Negative Control, Fluorescence, Real-time Polymerase Chain Reaction

Journal: Journal of Clinical Laboratory Analysis

Article Title: HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian province of China

doi: 10.1002/jcla.21573

Figure Lengend Snippet: Comparison of ALT and HBV – DNA Level in Patients with HB s A g(+) Anti‐ HB e(+) Anti‐ HB c(+)

Article Snippet: Quantization of HBV – DNA Real‐time fluorescent quantitative PCR method based Shenzhen PG kit (Shenzhen PG Biotech Company, Shenzhen, China) and Roche Lightcycler 480 (Roche Corporation, Basel, Switzerland) were used.

Techniques: Comparison, Concentration Assay

Journal: Journal of Clinical Laboratory Analysis

Article Title: HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian province of China

doi: 10.1002/jcla.21573

Figure Lengend Snippet: Comparison of ALT and HBV – DNA Level in Patients with HB sAg(+) HB eAg(+) Anti‐ HB c(+)

Article Snippet: Quantization of HBV – DNA Real‐time fluorescent quantitative PCR method based Shenzhen PG kit (Shenzhen PG Biotech Company, Shenzhen, China) and Roche Lightcycler 480 (Roche Corporation, Basel, Switzerland) were used.

Techniques: Comparison, Concentration Assay

Journal: Journal of Clinical Laboratory Analysis

Article Title: HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian province of China

doi: 10.1002/jcla.21573

Figure Lengend Snippet: Comparison of ALT and HBV – DNA Level of Patients with Different HB s A g Level

Article Snippet: Quantization of HBV – DNA Real‐time fluorescent quantitative PCR method based Shenzhen PG kit (Shenzhen PG Biotech Company, Shenzhen, China) and Roche Lightcycler 480 (Roche Corporation, Basel, Switzerland) were used.

Techniques: Comparison, Concentration Assay

Journal: Journal of Clinical Laboratory Analysis

Article Title: HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian province of China

doi: 10.1002/jcla.21573

Figure Lengend Snippet: Comparison of ALT and HBV – DNA Before and After the Concentration of HB e A g and HB s A g Changing

Article Snippet: Quantization of HBV – DNA Real‐time fluorescent quantitative PCR method based Shenzhen PG kit (Shenzhen PG Biotech Company, Shenzhen, China) and Roche Lightcycler 480 (Roche Corporation, Basel, Switzerland) were used.

Techniques: Comparison, Concentration Assay

Journal: Journal of Clinical Laboratory Analysis

Article Title: HBV serum markers of 49164 patients and their relationships to HBV genotype in Fujian province of China

doi: 10.1002/jcla.21573

Figure Lengend Snippet: Comparison of HB s A g, ALT , and HBV – DNA Level of Patients with Different Genotypes

Article Snippet: Quantization of HBV – DNA Real‐time fluorescent quantitative PCR method based Shenzhen PG kit (Shenzhen PG Biotech Company, Shenzhen, China) and Roche Lightcycler 480 (Roche Corporation, Basel, Switzerland) were used.

Techniques: Comparison, Concentration Assay